Based on the in-vitro potential of Ec.Chf, this was subjected to the in-vivo model experiment. The IC 50 values recorded for Ec.Chf were 112, 109, and 150 μg/ml against DPPH, ABTS, and H 2O 2 respectively. In antioxidant assays, Ec.Chf and Ec.Cr exhibited a considerable scavenging effect against all the free radicals. The rest of the samples showed moderate inhibition of α-glucosidase. The most active sample was Ec.Chf which revealed an IC 50 of 437 μg/ml in comparison to the standard acarbose (IC 50 25 μg/ml). In the α-glucosidase inhibition assay, a concentration-dependent inhibitory response was observed against the enzyme. Furthermore, comparative fingerprints of methanolic extract and chloroform fraction were also analyzed via High Performance Liquid Chromatography coupled with Diode Array Detector (HPLC-DAD). Moreover, different phytoconstituents were identified in the most active solvent fraction by GC-MS analysis. The antioxidant activity was determined by using different free radicals -6-sulfonic acid (ABTS), and hydrogen peroxide (H 2O 2)]. Both in-vitro and in-vivo assays have been carried out for antidiabetic assays.
Based on the medicinal importance of the genus Eryngium and unexplored nature of Eryngium caeruleum, we have evaluated its antidiabetic and antioxidant potentials. Natural-based drugs are believed to be safe, effective and economical.